CytExpert Software for CytoFLEX

Intuitive workflow capabilities for complex flow cytometric applications setup Intuitive graphic interface for novice and flow experts alike

The CytExpert software is a full-feature software package that controls the instrument's operation, collection of experiment data, and analysis of the results. It was developed to allow novice to experienced flow cytometrists learn to operate the CytoFLEX Flow Cytometer quickly and to allow new users to confidently set up experiment based protocols and export publication quality data.

Request a free copy of CytExpert 2.1


  • Processor: 4th Gen Intel TM Core i3 (3MB Cache, 2.90 GHz) equivalent or above
  • Operating system: Windows 7, 8, 10 Professional, 64 bit
  • Memory: 4 GB RAM or above
  • Hard disk space: At least 1 G free space for the disk of the experiment for analysis. Recommend 10 G for data acquisition.
  • Display: 1920 x 1080 resolution for optimal display
  • USB Port: USB 2.0 or above for data acquisition

User Management and Electronic Records

The Default installation requires no user login. For multi-user instruments, the User Management installation requires user login and contains features for role management. Electronic Records Management installation provides tools that facilitate compliance with 21 CFR Part 11, Electronic Records and Electronic Signatures. This includes controls for user identification, permissions, electronic signatures, data integrity, operation and experiment logs and audit trails.

CytExpert Compensation FigureDue to the highly reproducible semiconductor process, the fluorescence intensities measured on the CytoFLEX Platform are linear to the corresponding detector gain settings. Due to the gain linearity of the semiconductor, the compensation matrix obtained at one gain setting can be used for actual experiments at different gain settings. Compensation matrix elements obtained at different gain settings can be mixed together to form a full matrix, the Compensation Library.

Standardization allows operators to use the QC beads to set target values for different applications and calibrate the gain settings automatically. The Standardization Target Library stores and allows users to retrieve a variety of application target files which are linked to the QC bead lot numbers.

The CytExpert API (Application Programming Interface) allows external software can also run methods and control the plate loader using integrating into existing automation systems as well.

A new analysis tool to facilitate data interpretation when using the plate loader mode was added with version 2.0. Heat Maps with up to six parameters are available. Each experiment can include several heat maps. Values can be displayed in single parameter heat maps.

For research use only. Not for use in diagnostic procedures.