You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cl...

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve...
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The only RT-PCR kit able to offer both TA/UA cloning and high fidelity in a single system.
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activi...

Catalog Number: (CA95040-312L)
Supplier: Cytiva
Description: T4 DNA Ligase


Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency a...

Catalog Number: (CA99900-690)
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The DNA Ligation Kit includes T4 DNA ligase for highly efficient ligations.


Catalog Number: (CA99902-306)
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage ...


Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This e...

Catalog Number: (CA80031-486)
Supplier: MilliporeSigma
Description: The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of ...


Supplier: SGI - DNA Synthetic Genomics
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion

SDS

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotid...

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature
Supplier: New England Biolabs (NEB)
Description: T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Catalog Number: (CA200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleoti...


Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hyd...

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