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You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Ward's® Plasmid DNA for Transformations

Supplier: Edvotek
Description: These plasmids allow you to perform bacterial transformations, conferring new antibiotic resistance, as a visual way of demonstrating gene transfer.

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pJY2 Plasmid, Enzo Life Sciences

Catalog Number: (89157-406)
Supplier: Enzo Life Sciences
Description: pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
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Expression vectors, pGEX

Supplier: Cytiva
Description: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

SDS Certificates

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pESC Yeast Epitope Tagging Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast <i>S. cerevisiae </i>and each vector contains GAL1 and GAL10 yeast promoters in opposing orientations.

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pCMV-Script Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

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pCMV-Tag Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

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pSG5 and pMC1Neo Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector.

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Lambda Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: Lambda DASH II and Lambda FIX II vectors offer traditional genomic cloning of 9 to 23 kB fragments.

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Vitality hrGFP II Mammalian Expression Vectors, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: Vitality hrGFP II Mammalian Expression Vectors support a variety of expression configurations, thus providing ideal expression options for each specific application.

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AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

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Polyadenylic acid (poly-A)

Supplier: Cytiva
Description: Polyadenylic acid (poly-A) is a single stranded RNA homopolymer.

SDS

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Lambda ZAP II Vector Kits, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The Lambda ZAP II vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems.

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LacSwitch II, Vectors, Agilent Technologies

Catalog Number: (CA95040-814)
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.
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InterPlay Adenoviral TAP Systems, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

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Lambda gt11 Vector Kits, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: For use in the construction of cDNA libraries.

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Lambda ZAP Vector Kits, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Description: The ZAP Express and the Lambda ZAP-CMV vectors combine the high efficiency of lambda vector systems with the versatility of plasmid systems.

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