CJ236 Electrocompetent Cells, Biosearch TechnologiesSupplier: LUCIGEN CORPORATION CA
89260-192EA 595.03 CAD89260-192 89260-190
CJ236 Electrocompetent Cells, Biosearch Technologies
This cell line was created by adding the pCJ105 plasmid to BW313 cells, and is ideally suited to the Kunkel directed mutagenesis method
This method relies on the cells producing plasmid DNA containing some uracil residues. The dut- and ung- mutations prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. Note that these cells contain an F’ which confers resistance to Chloramphenicol at low levels.