Anti-TRPV1 Guinea Pig Polyclonal Antibody

Supplier: GeneTex

89366-698EA 479.51 CAD
Anti-TRPV1 Guinea Pig Polyclonal Antibody
Guinea Pig polyclonal to VR1 C-terminus antibody

For IHC: Use at a dilution of 1:1,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) 4% paraformaldehyde fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Note: Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.

Type: Primary
Antigen: TRPV1
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Guinea Pig
Reactivity: Human
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