Anti-IL4 Goat Polyclonal Antibody

Supplier: GeneTex
89355-004EA 928.09 CAD
Anti-IL4 Goat Polyclonal Antibody
Goat polyclonal to IL4

ELISA: Use at a concentration of 0.5 - 1 ?g/ml, this detects 0.3 ng/well of recombinant mouse IL4Neut: Use at a concentration of 0.1 - 0.3 ?g/ml. WB: Use at a concentration of 0.1 - 0.2 ?g/ml, this detects recombinant mouse IL4 at 2 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 20 kDa. Anti-Mouse Interleukin 4 (IL 4) is tested for its ability to neutralize the bioactivity of recombinant mouse interleukin 4 in a cell proliferation assay using a IL 4 responsive murine T helper cell line, HT2. In this bioassay, recombinant mouse IL4 is preincubated with various dilutions of the antibody for 1 hour at 22°C in a 96-well plate. HT-2 cells are added to each well. The total volume of 100 ?l, containing antibody, recombinant mouse IL4 at 7.5 ng/ml, and cells at 1 x 10^5 cells/ml, is incubated for 48 hours at 37 °C in a 5% CO2 humidified incubator and then pulsed for the last 4 hours with ^3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA is measured. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: IL4
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Goat
Isotype: IgG
Reactivity: Mouse
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