Anti-OPG Goat Polyclonal Antibody

89354-050EA 653.43 CAD
Anti-OPG Goat Polyclonal Antibody
Goat polyclonal to Osteoprotegerin

ELISA: Use at a concentration of 0.5 - 1 ?g/ml, this detects a limit of ~0.16 ng/well of recombinant human OPG. Neut: Use at a concentration of 0.2 - 0.7 ?g/ml, this will neutralize 50% of the bioactivity due to 0.1?g/ml recombinant human OPG using the TRAIL sensitive mouse L929 cytolytic assays. WB: Use at a concentration of 0.1 - 0.2 ?g/ml, this is determined using human OPG at 5 ng/lane under non-reducing and 25 ng/ml under reducing conditions. Predicted molecular weight: 48 kDa. To measure the ability of the antibody to neutralize the bioactivity of human OPG, soluble recombinant human OPG was incubated with various concentrations of the antibody in media containing actinomycin D for 60 minutes at 37 °C. Cross-linked recombinant human TRAIL was then added and incubated for 30 minutes. Following preincubation, the mixture was added to confluent cultures of L929 cells in 96 well microplates. The assay mixture over a monolayer of L929 cells contained antibody at concentrations of 0.001-10 ?g/ml, recombinant human OPG at 0.1 ?g/ml, actinomycin D at 1 ?g/ml, cross-linked human TRAIL at 20 ng/ml in a total volume of 150 ?g/ml. The plate was incubated at 37 °C for 24 hours in a humidified CO2 incubator. The media was removed, the cells were fixed with 5% formaldehyde, and then stained with crystal violet. After dissolving the dye with 33% acetic acid, the optical density was read at 540 nm. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: OPG
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Goat
Isotype: IgG
Reactivity: Human
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