Anti-P2RX3 Rabbit Polyclonal Antibody

Supplier: Genetex

89366-688EA 467.74 CAD
Anti-P2RX3 Rabbit Polyclonal Antibody
Rabbit polyclonal to P2X3 antiserum

Immunohistochemistry 1:1000 Immunocytochemistry 1:1000 Western Blotting 1:1000 Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Western Blotting: Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 4?C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with primary antiserum (1:500 in the same buffer overnight at 4?C. Membranes were rinsed and incubated with horseradish peroxidase conjugated secondary antibody for 1 hour at room temperature. Following rinsing, the membranes were processed using enhanced chemiluminescence.

Type: Primary
Antigen: P2RX3
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human
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