Mouse monoclonal [100 / 2] to alpha Adaptin
WB: Use at a dilution of 1/200. Reacts in bovine and rat brain preparations, with the 105 and 110 ?-subunits of adaptor complex AP2. The antibody reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. The antibody stains the intact alpha adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of alpha fragments obtained by trypsin cleavage of the alpha subunits molecules. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Reactivity: Human, Rat, Bovine