Anti-HAT1 Rabbit Polyclonal Antibody

Supplier: GeneTex
89359-214EA 565.23 CAD
Anti-HAT1 Rabbit Polyclonal Antibody
The basic repeating unit of eukaryotic chromatin is the nucleosome, which consists of 147 bp of DNA wrapped around an octameric protein core formed of two molecules each of histones H2A, H2B, H3, and H4. These core histones are required for nuclear packaging and DNA organization as well as for regulating various cell processes. Acetylation is the most extensively studied posttranslational histone modification. Histone acetyltransferases (HATs) are members of a superfamily of enzymes that transfer the acetyl moiety from acetyl-coenzyme A cofactor onto one or more epsilon-amino groups of lysines contained in the extended N-terminal tail domains of core histone proteins. Type A HATs are located in the nucleus, and many of them play a role as transcriptional coactivators. Type B HATs, traditionally thought to locate to the cytoplasm, acetylate nascent cytoplasmic histones prior to chromatin assembly. HAT1, a type B HAT, has been identified in several species ranging from yeast and maize to human. Native human HAT1 is a ~100 kDa heterodimer consisting of a catalytic subunit (46 kDa) and a corehistone- binding subunit (Rbap46/Hat2) that greatly stimulates the acetyltransferase activity of the holoenzyme. Human HAT1 rapidly diacetylates soluble non-nucleosomal histone H4 at lysine-5 and lysine-12 with similar efficiency. It also acetylates, albeit to a lesser extent, histone H2A at lysine 5. These sites are distinct from those modified in transcriptional regulation. The newly synthesized acetylated histones interact with cellular proteins such as chromatin assembly factor 1 (CAF-1) to form complexes. Following deposition of the acetylated histones into chromosomes they are rapidly deaceylated. HAT1 is present in S-phase cell nuclei and in the cytoplasm. HAT1 may be involved in processes of chromatin assembly, chromatin repair, maintenance of specific heterochromatin acetylation pattern, and telomeric silencing.

WB: Use at a dilution of 1/2500. Detects a band of approximately 46 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: HAT1
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse
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