Anti-Polb Mouse Monoclonal Antibody [clone: 61]

Supplier: GeneTex
89351-646EA 607.25 CAD
Anti-Polb Mouse Monoclonal Antibody [clone: 61]
DNA polymerase beta comprises an amino-terminal 8 kD domain and a carboxy-terminal 31 kD domain. DNA Polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For IHC-P: Use at a dilution of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For WB: Use at an assay dependent diluiton. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Type: Primary
Antigen: Polb
Clonality: Monoclonal
Clone: 61
Conjugation: Unconjugated
Host: Mouse
Isotype: IgG2a
Reactivity: Human
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