Anti-IFNG Goat Polyclonal Antibody

Supplier: GeneTex
89367-186EA 740.67 CAD
Anti-IFNG Goat Polyclonal Antibody
Goat polyclonal antibody to IFN gamma

ELISA: Use at a concentration of 0.5 - 1 ?g/ml to detect <0.6 ng/well of recombinant, human IFN gamma. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 ?g/ml to detect 5 ng/lane of recombinant, human IFN gamma under reducing and non-reducing conditions. Predicted molecular weight: 19.3 kDa. IFN gamma is tested for its ability to neutralize the biological activity of rhIFN gamma on HeLa cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhIFN gamma that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 5 ng/ml rhIFN gamma was mixed with various dilutions of the antibody and the antigen-antibody mixture was added to confluent cultures of HeLa cells in a 96-well plate. The assay mixture was incubated at 37 °C for 20-24 hours in a humidified CO2 incubator. After incubation, the medium was aspirated from all wells and encephalomyocarditis virus (EMCV) was added to each test well. The 96-well plate was incubated for an additional 20-24 hours. The cells were fixed and examined for cytopathic effect by measurement of optical densities in a microplate reader at 540 nm. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: IFNG
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Goat
Isotype: IgG
Reactivity: Human
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