Anti-MMP25 Rabbit Polyclonal Antibody

GTX11652
89358-652EA 472.59 CAD
89358-652
Anti-MMP25 Rabbit Polyclonal Antibody
Matrix Metalloproteinase 25, also known as membrane type matrix metalloproteinase 6 and leukolysin, was initially cloned from human leukocytes and found to be specifically expressed by peripheral blood leukocytes. The expression of MMP25 (leukolysin) at the mRNA level appears to be localized to neutrophils. MMP25 was also found in lung, spleen, brain tumors, and several tumor cell lines. Like the other membrane-type metalloproteinases, MMP25 is associated with the cell membrane, but is anchored to the cell surface via a GPI (glycosyl-phosphatidyl inositol) attachment, rather than through a transmembrane domain. MMP25 is proteolytically active and truncated forms digest gelatin in zymograms. Like the other MT MMPs, MMP25 has a furin cleavage site, where prohormone convertases can activate the zymogen. MMP25 can be shed from the from the cell surface, into the culture media under stimulatory conditions. MMP25 is expressed in brain tumors and may facilitate tumor progression through its ability to activate progelatinase A at the cell membrane in colon carcinomas or brain tumors. MMP25 may also play a role in the cellular migration and invasion of the extracellular matrix and basement membranes. The MMP25 gene maps to human chromosome 16p13.3.

WB: Use at an assay dependent dilution. A minimum working dilution of 1/1000 is recommended using a cell lysate, an alkaline phosphatase conjugated secondary antibody, and BCIP/NBT as the substrate. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. By blotting against the reduced protein, the antibody identifies bands at 65 kDa, as well as activation/breakdown products. Treatment of cells with concanavolin A or the phorbol ester, TPA,stimulates production of MMP25 in some cell types, and the enzyme can be recovered in cell lysates. Note: Higher antibody dilutions may be necessary for non-human samples. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: MMP25
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human
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