Anti-MMP28 Rabbit Polyclonal Antibody

Supplier: GeneTex
89358-822EA 940.34 CAD
Anti-MMP28 Rabbit Polyclonal Antibody
The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc-endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. MMPs contain the motif His-Glu-X-X-His (X represents any amino acid) that binds zinc in the catalytic site, as well as another zinc molecule and two calcium molecules structurally. They fall within the matrixin subfamily and are EC designated 3.4.24.x. This group also contains astacin, reprolysin, and serralysin, as well as other more divergent metalloproteinases. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown.

WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. By immunoblotting against the reduced protein, the antibody identifies bands at 62 kDa and 58 kDa, as well as breakdown products at 50 kDa, 48 kDa, and 46 kDa. It also detects a 70 kDa band in mouse and rat tissues which probably represents the larger proform. Note: Higher antibody dilutions may be necessary for non-human samples. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: MMP28
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human
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