Anti-PDE11A4 Rabbit Polyclonal Antibody

Supplier: GeneTex
89367-376EA 719.21 CAD
Anti-PDE11A4 Rabbit Polyclonal Antibody
Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. Recently a new and distinct member (PDE11) of cyclic nucleotide family has been identified and cloned. The PDE11A exhibit 50% amino acid homology with the catalytic domain of the all other PDEs. The PDE11A resembles most to the PDE5 family members but has distinct biochemical properties than PDE5. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55 to 56 kDa). The PDE11A has 4 splice varaints (PDE11A1, A2, A3 and A4) and has an N terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10 this potentially represent an allosteric cGMP and other small signaling molecules. PDE11A exhibits properties of a dual substrate PDE. The deduced amino acid sequence of the longest form of rat PDE11A splice variant, RNPDE11A4, was 94% identical with that of the human variant (HSPDE11A4). The Km for for cAMP and cGMP were similar for all PDE11 variants (1.3 to 1.6 and 2.1 to 3.9 microM, PDE11A2, A3 and A4, respectively), the Vmax values differed significantly (RNPDE11A4 >> RNPDE11A2 > RNPDE11A3). Human PDE11A variants also displayed very similar Km values and significantly different Vmax values (human PDE11A4 >> A3 > A3 >> A1). The Vmax values of HSPDE11A4 for cAMP and cGMP were at least 100 times higher than those of HSPDE11A1. These observations indicate unique characteristics of PDE11A splicing variants.

ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. Detects a band of approximately 113 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: PDE11A4
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human
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