pJY2 Plasmid, Enzo Life Sciences

Supplier: Enzo Life Sciences
DW8720-0005
89157-406EA 538.67 CAD
89157-406
pJY2 Plasmid, Enzo Life Sciences
Vectors, Plasmids, Libraries and Inserts
pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.

  • Liquid
  • Prepared in 10 mM TRIS, pH 8.0, containing 1mM EDTA
  • Store at –20 °C

The AGA arginine codon is rarely used in E. coli but is common in eukaryotic genes. The AGG arginine codon is also infrequently utilized in E. coli. As tRNAUCUArg decodes AGG when over-expressed, pJY2 is also likely to ameliorate problems associated with the expression of genes containing AGG codons.
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