Ficoll PM400, Cytiva

Supplier: Cytiva

Supplier: Cytiva

About this item

Ficoll® PM400 is a synthetic neutral, highly branched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It is used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilising agent and as a preparation medium for isolating mononuclear cells. It is useful for separating cells that are sensitive to centrifugation. Ficoll™ PM400 can also be used in other applications, such in defined culture media, nucleic acid hybridisation, electrophoresis, and immunological studies.

Concentrations of up to 50% (w/v) covering densities of up to 1,2 g/ml can be achieved
Better osmotic properties than sucrose
Preserves functional and morphological integrity
Does not penetrate biological membranes
Serves as the raw material for preparation of Ficoll-Paque gradients

Specifications

Catalog No:
CA95021-194L
CA95038-162L
CA95021-196L

Endotoxin activity:
0.12 EU/mg (Max.)
0.12 EU/mg (Max.)
0.12 EU/mg (Max.)

Molecular weight:
~ 3 × 105 [Mr] Min.; ~ 5 × 10~ 5 [Mr] Max.
~ 3 × 105 [Mr] Min.; ~ 5 × 10~ 5 [Mr] Max.
~ 3 × 105 [Mr] Min.; ~ 5 × 10~ 5 [Mr] Max.

Radius:
~ 10 nm (Stokes Radius)
~ 10 nm (Stokes Radius)
~ 10 nm (Stokes Radius)

Density:
1.2 g/ml (Max.)
1.2 g/ml (Max.)
1.2 g/ml (Max.)

Viscosity:
Intrinsic Viscosity: ~ 17 ml/g;
Relative Viscosity: 5 ηr at 20°C: 10% (w/v),
20 ηr at 20°C: 20% (w/v),
60 ηr at 20°C : 30% (w/v),
180 ηr at 20°C : 40% (w/v),
600 ηr at 20°C : 50% (w/v)
Intrinsic Viscosity: ~ 17 ml/g;
Relative Viscosity: 5 ηr at 20°C: 10% (w/v),
20 ηr at 20°C: 20% (w/v),
60 ηr at 20°C : 30% (w/v),
180 ηr at 20°C : 40% (w/v),
600 ηr at 20°C : 50% (w/v)
Intrinsic Viscosity: ~ 17 ml/g;
Relative Viscosity: 5 ηr at 20°C: 10% (w/v),
20 ηr at 20°C: 20% (w/v),
60 ηr at 20°C : 30% (w/v),
180 ηr at 20°C : 40% (w/v),
600 ηr at 20°C : 50% (w/v)