HiTrap Columns, rProtein A FF, Cytiva
Supplier: Cytiva
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About this item
HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
- Quick, convenient preparative purification of monoclonal antibodies
- Well-established protein A-based resin used in many approved MAb processes
- Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design
Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
Specifications
- Catalog No:
- CA95056-088L
- CA95056-090L
- CA95056-008L
- CA95055-868L
- Max. pressure:
- 5 bar [0.5 MPa] (70 psi)
- 5 bar [0.5 MPa] (70 psi)
- 5 bar [0.5 MPa] (70 psi)
- 5 bar [0.5 MPa] (70 psi)
- pH range:
- 2 - 9
- 2 - 9
- 2 - 9
- 2 - 9
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifications
Related Information
Cytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
17507901_DATA FILE 1.pdf 17507901_DATA FILE.pdf 17507902_DATA FILE 1.pdf 17507902_DATA FILE.pdf 17508001_DATA FILE 1.pdf 17508001_DATA FILE.pdf 17508002_DATA FILE.pdf
Datasheet
17507901_HAND BOOK 1.pdf 17507901_HAND BOOK 2.pdf 17507901_HAND BOOK 3.pdf 17507901_HAND BOOK.pdf 17507901_INSTRUCTIONS.pdf 17507902_HAND BOOK 1.pdf 17507902_HAND BOOK 2.pdf 17507902_HAND BOOK.pdf 17507902_INSTRUCTIONS.pdf 17508001_HAND BOOK 1.pdf 17508001_HAND BOOK 2.pdf 17508001_HAND BOOK.pdf 17508001_INSTRUCTIONS.pdf 17508002_HAND BOOK 1.pdf 17508002_HAND BOOK.pdf 17508002_INSTRUCTIONS.pdf
Manual
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