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undefined | Nucleic Acid Purification Kits and Reagents
Catalog #: 89492-616
Supplier:  Avantor
Catalog #: 89492-616
Supplier:  Avantor
Discontinued
Product has been discontinued. However we found similar alternative products.
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YEAST GENOMIC DNA PURIFICATION KIT Catalog #CA89492-616

Product CA89492-616 is similar to 89492-616 but manufacturer and specifications may vary.

YEAST GENOMIC DNA PURIFICATION KIT Catalog #89492-616

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gYEAST GENOMIC DNA REAGENT KIT 300 PREPS Catalog #89493-622

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Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

  • • State issued document with your organization's Federal Tax ID Number
  • • State issued document with your organization's Resale Tax ID Number
  • • City or County issued Business License
  • • State Department of Health Services License
  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.

About this item

Yeast Genomic DNA Purification Kit yields high molecular weight DNA from yeast using a non-enzymatic lysis buffer and no glass beads

  • Rapid purification yields high quality, high molecular weight DNA
  • No enzymes, glass beads or freeze thawing for cell lysis
  • Method is phenol, chloroform and column-free
  • Easily scale purification as needed

The protocol is simple, requiring about 1 hour to complete, and may be scaled for large or small samples. The purification process begins with lysis of the yeast cell wall with optional RNase A treatment, and is followed by protein precipitation and then DNA precipitation. Unlike many conventional yeast genomic DNA purification methods, no phenol, chloroform or columns are required. The kit, which includes Lysis Buffer, Protein Precipitation Solution, RNase A, 10 mg/ml and Resuspension Buffer, works with liquid cultures as well as colonies. The purified DNA yields vary for different yeast strains and is also dependent on cell density. The kit is optimized for Saccharomyces cerevisiae, with an average yield of 1.5 µg DNA per 5 x 107 cells as measured by VWR’s Fluorescent DNA Quantitation Kit. High quality DNA obtained with the Yeast Genomic DNA Purification Kit is compatible downstream applications, such as, PCR, restriction enzyme digestions and Southern blotting.

Ordering: Kit includes Yeast Lysis Solution; Ribonuclease A (RNase A); Protein Precipitation Solution; TE, pH 8.0.
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