About this item
The Expresso Rhamnose Cloning & Protein Expression System clones a PCR amplified gene in an effortless afternoon, and express recombinant protein the next day.
- Five-second, directional, enzyme-free PCR cloning, 90% recombinants
- Single competent cell host strain for both cloning and expression
- Tighter expression control with tunable Rhamnose (rhaPBAD) promoter
- High throughput format friendly with autoinduction reagents
It contains pre-processed pRham N-His and/or pRham C-His Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are N-His and/or C-His Positive Control Insert DNAs, transformation positive control pUC DNA, and forward and reverse PCR primers to confirm clones.
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