Anti-EGF Rabbit Polyclonal Antibody

Supplier: Prosci
XP-5119
10098-416EA 786.51 CAD
10098-416
Anti-EGF Rabbit Polyclonal Antibody
Antibodies
Neutralization:<br> To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 ?g/mL of this antibody is required. <br><br>ELISA:<br><br>To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. <br><br>Sandwich: To detect hEGF by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. <br><br>Western Blot:<br>To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: EGF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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