Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat GRO?/MIP-2 (100 ng/mL), a concentration of 3.0 - 5.0 µg/mL of this antibody is required. ELISA: To detect Rat GRO?/MIP-2 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat GRO?/MIP-2. Sandwich: To detect Rat GRO?/MIP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GRO?/MIP-2 (XP-5149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GRO?/MIP-2. Western Blot: To detect Rat GRO?/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GRO?/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-Rat GRO?/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GRO?/MIP-2 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Cxcl2
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Rat