Anti-IL13 Rabbit Polyclonal Antibody (Biotin)

Supplier: Prosci
10097-864EA 786.51 CAD
Anti-IL13 Rabbit Polyclonal Antibody (Biotin)
L13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58% amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30% sequence homology and have similar biological functions.

ELISA: To detect mIL-13 by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-13. Sandwich: To detect mIL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-13 (XP-5168) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13. Western Blot: To detect mIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-mIL-13 specific antibody was purified by affinity chromatography and then biotinylated.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: Il13
Clonality: Polyclonal
Conjugation: Biotin
Host: Rabbit
Reactivity: Mouse
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