Anti-rpoH Rabbit Polyclonal Antibody (Biotin)

Supplier: ProSci
XP-5212BT
10097-500EA 786.51 CAD
10097-500
Anti-rpoH Rabbit Polyclonal Antibody (Biotin)
Antibodies
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

ELISA: To detect hLeptin by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLeptin. Sandwich: To detect hLeptin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Leptin (XP-5212) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-hLeptin specific antibody was purified by affinity chromatography and then biotinylated.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: rpoH
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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