Anti-CCL3 Rabbit Polyclonal Antibody (Biotin)

Supplier: ProSci
XP-5233BT
10097-542EA 786.51 CAD
10097-542
Anti-CCL3 Rabbit Polyclonal Antibody (Biotin)
Antibodies
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

ELISA: To detect rat MIP-1-? by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat MIP-1-?. Sandwich: To detect Rat MIP-1? by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MIP-1? (XP-5233) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1?. Western Blot: To detect rat MIP-1-? by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MIP-1-? is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-rat MIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: Ccl3
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Rat
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