Anti-CCL3 Rabbit Polyclonal Antibody

Supplier: ProSci
XP-5234
10097-538EA 786.51 CAD
10097-538
Anti-CCL3 Rabbit Polyclonal Antibody
Antibodies
MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1-alpha (10 ng/mL), a concentration of 2.0 - 5.0 µg/mL of this antibody is required. ELISA: To detect mMIP-1-alpha by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1-alpha. Sandwich: To detect hMIP-1? by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-1? (XP-5234Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1?. Western Blot: To detect mMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography employing immobilized mMIP-1-alpha matrix.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: Ccl3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Mouse
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