Anti-PDGFA Rabbit Polyclonal Antibody

Supplier: ProSci
XP-5254
10097-762EA 786.51 CAD
10097-762
Anti-PDGFA Rabbit Polyclonal Antibody
Antibodies
Neutralization:<br> To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (10.0 ng/mL), a concentration of 0.17 - 0.19 ?g/mL of this antibody is required. <br><br>ELISA:<br><br>To detect hPDGF-AA by direct ELISA (using 100 ?L/well antibody solution) a concentration of at least 0.5 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-AA. <br><br>Sandwich:<br><br>To detect hPDGF-AA by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PDGF-AA (XP-5254Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA.<br><br>Western Blot:<br><br>To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: PDGFA
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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