ELISA:<br><br>To detect mSDF-1? by direct ELISA (using 100 ?L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ?g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mSDF-1?.<br><br>Sandwich:<br><br>To detect mSDF-1? by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine SDF-1? (XP-5270) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSDF-1?. <br><br>Western Blot:<br>To detect mSDF-1? by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSDF-1? is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Cxcl12
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Goat
Isotype:
Reactivity: Mouse