ELISA:<br><br>To detect hsRANKL by direct ELISA (using 100 ?L/well antibody solution) a concentration of at least 0.5 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsRANKL.<br><br>Sandwich:<br><br>To detect hsRANKL by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sRANKL (XP-5273Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL. <br><br>Western Blot:<br>To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: TNFSF11
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human