Anti-Dnm1 Sheep Polyclonal Antibody

Supplier: ProSci
10075-654EA 1078.7 CAD
Anti-Dnm1 Sheep Polyclonal Antibody
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Dynamin antibody for phospho-Ser778 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser778) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for WB.

Affinity Purified

100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.

Aliquot and store at -80°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: Dnm1
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Sheep
Reactivity: Human, Mouse, Rat
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