Anti-GLRA1 Rabbit Polyclonal Antibody

Supplier: ProSci
10075-540EA 1078.7 CAD
Anti-GLRA1 Rabbit Polyclonal Antibody
Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult).

Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB). Rabbit anti-Glycine Receptor recognizes glycine receptor ?1 and ?2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Affinity Purified

Reconstitute in 50 μl PBS. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: Glra1
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Reactivity: Rat
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