96-Well Ni-NTA HisPrime Plates, 5PRIME

2400730 2400720
10052-328EA 286.25 CAD
10052-328 10052-326
96-Well Ni-NTA HisPrime Plates, 5PRIME
Ni-NTA HisPrime plates provide Ni-NTA in a solid-phase, multiwell format for 6xHis tag assay of proteins or other molecules

  • High sensitivity by uniform orientation of native His-tagged proteins bound to the Ni-NTA coated surface
  • High selective binding for testing proteins even in crude lysates and diluted solutions
  • Rapid and easy procedure for protein screening and assays

Can be used for:
Quantifying 6xHis-tagged molecules
Establishing ELISA or RIA procedures or diagnostic assays
Studying molecular interactions
Screening expression clones, engineered enzymes, pharmaceuticals, antibodies, or serum samples

Tagged molecules are not denatured and are bound to the plate in a uniform orientation. They are conformationally active while remaining available to detection reagents (antibodies or any interacting molecule). Ni-NTA HisPrime plates exhibit increased sensitivity compared to polystyrene plates, where molecules are randomly adsorbed in non uniform orientations and can be denatured by immobilisation. The plates can be used to assay proteins in crude cell lysates and dilute solutions as 6xHis-tagged proteins are selectively bound to the Ni-NTA coated surface. Ni-NTA HisPrime plates are available in either a transparent format for colorimetric assays, or an opaque, white format for luminescence- and fluorescence-based assays. They are compatible with standard multichannel pipettes as well as automated plate washers and readers. Stable in dry, room temperature storage for up to one year. Small peptides: ~ 20 pmol/well. Proteins: 2–10 pmol/well (10 pmol of a 25 kDa protein corresponds to 250 ng). Maximum volume per well: 300 µl.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR