RealMasterMix™ Fast Probe Kits, QuantaBio

2200770 2200760 2200740 2200790 2200780 2200750
10052-290EA 2677.29 CAD
10052-290 10052-288 10052-284 10052-294 10052-292 10052-286
RealMasterMix™ Fast Probe Kits, QuantaBio
  • High specificity - stringent, antibody-mediated hot-start eliminates non specific primer annealing
  • Excellent sensitivity - reliable detection of even low-abundance targets (down to 1 copy)
  • Fast - real-time PCR results in about 30 minutes
  • Flexible - optimized for any cycler with fast-cycling and standard protocols
  • Easy to use master mix - RealBlue dye for pipetting and mixing control

RealMasterMix™ Fast Probe kits include a 2X concentrated, ready to use master mix for very fast real-time quantification of DNA and cDNA targets using sequence-specific probes. There is no compromise in sensitivity, specificity, dynamic range, or PCR efficiency. Only primers and template must be added in addition. Three different kits are available with the correct amount of reference dye optimized for different real-time cyclers. The light blue colour of the RealBlue dye simplifies reaction assembly in white or clear plates and helps to minimize pipetting and mixing errors.

Maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast-, or conventional, cycling protocols with sequence-specific probes are achieved through a unique combination of proprietary buffer, stabilizers, and SpeedAB Taq Polymerase.

The different RealMasterMix Fast Probe Kits are optimized for distinct cycler requirements and are suitable for standard and fast cyclers. On fast instruments, RealMasterMix Fast Probe Kits deliver PCR results in less than half the time of standard real-time PCR reagents, whilst maintaining equivalent PCR performance.

RealMasterMix Fast Probe Kits can also be used on standard cyclers using a faster protocol to decrease the PCR run time. For fast cycling, existing primer sets can be used without reoptimization. It is crucial to match the appropriate qPCR reagent with the specific instrument, since different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations.
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