Anti-RB1 Rabbit Monoclonal Antibody [clone: 14H7L14]

Supplier: Pierce Biotechnology
CAPI701059EA 1564.82 CAD
Anti-RB1 Rabbit Monoclonal Antibody [clone: 14H7L14]
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology. ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A. ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Rb, also known as Retinoblastoma, is a tumor suppressor protein that keeps uncontrolled growth and division in check. Rb belongs to the Pocket Protein family whose members have a pocket for binding proteins. It is nuclear protein which is widely expressed in retinal cells. It is inactivated by phosphorylation and activated by dephosphorylation. Phosphorylation of serine 249 and threonine 252 is catalyzed by Cyclin D-cdk4. In humans, the protein is encoded by the RB1 gene located on the q arm of chromosome 13.

Recommended Dilutions: Western Blot: 0.1-2 ?g/ml, Immunofluorescence: 1-3 ?g/ml, Immunocytochemistry: 1-3 ?g/ml, Flow Cytometry: 1-3µg x 10^6 cells

Type: Primary
Antigen: RB1
Clonality: Monoclonal
Clone: 14H7L14
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human
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