About this item
PromoKine's PCR Mycoplasma Test Kit II has been designed to detect sensitively and specifically the presence of mycoplasmas that are contaminating biological materials.
- Unique 5x master mix
- Direct loading of PCR products onto an agarose gel
- Detects all mycoplasma commonly found in cell cultures
PromoKine's PCR Mycoplasma Test Kit II has been designed to detect sensitively and specifically the presence of mycoplasmas that are contaminating biological materials such as cultured cells.
The kit includes a unique 5x master mix that contains all the ingredients required for PCR. No prior preparations are required for PCR, other than the sample to be tested. The reaction mix contains a precipitant allowing for direct loading of PCR products onto an agarose gel. After performing agarose gel electrophoresis, positive samples will yield a 270 bp fragment.
The test takes approximately five hours to obtain clear results and detects all mycoplasma commonly found in cell cultures. The primers have been designed to detect those mycoplasma species responsible for most contaminations in cell cultures (including Acholeplasma) and were tested to be specific for mycoplasma DNA only, not reacting with animal or bacterial DNA.
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