Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt), Pierce™

Supplier: Thermo Scientific

Synonyms: Hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid sodium saltSulfo NHSHydroxy-2,5-dioxopyrrolidine-3-sulphonicacid sodium saltSulpho-N-HydroxysuccinimideSulfo-N-Hydroxysuccinimide

24525 24510 24525 24510
CAPI24510EA 851.16 CAD
PI24525 PI24510 CAPI24510 CAPI24525
Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt), Pierce™
Sulfo-NHS (N-Hydroxysulfosuccinimide sodium salt)

Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.


  • Improve efficiency of EDC coupling reactions
  • Water-soluble and membrane-impermable
  • Activate nanoparticles with amine-reactive Sulpho-NHS esters
  • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently


Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₄H₄NNaO₆S
MW: 217.13 g/mol
MDL Number: MFCD00043100
CAS Number: 106627-54-7

Order Now

Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Carbodiimide
Spacer Short
Form Powder
Quantity 500 mg
Type Crosslinker
Solubility Water

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR