siRNA Cloning Vector (pGB), BiovisionSupplier: BioVision
10013-304EA 446.18 CAD10013-304
siRNA Cloning Vector (pGB), Biovision
Vectors, Plasmids, Libraries and Inserts
Vector containing the human U6 RNA polymerase III promoter and BamH I sites for directional cloning.
- Application- The pGB siRNA vectors can be transfected into mammalian cells using Lipofectamine (Invitrogen).
pGB expression vectors contain the human U6 RNA polymerase III promoter, which directs constitutive, high-level expression of short RNA transcripts in many cells. Each vector also contains the neomycin/kanamycin-resistance gene to provide kanamycin resistance in bacteria and the G418 resistance in mammalian cells. The pGB vector is used to clone your own insert. The vector contains two unique restriction sites, BamH I and Xba I for directional cloning. The pGB Negative Control vector contains a insert that does not have significant homology to mammalian genes expressed in human, mouse, and rat, and it can be used as a negative control for pGB-Caspase siRNA vectors. The pGB Caspase siRNA vectors contain the siRNA inserts designed to suppress the expression of each caspase individually