Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them. Switch easily between top and bottom reading modes for solution- and cell-based assays.

• Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
• Eliminate the identification, purchase, and changing out of filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
• Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
• Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
• Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well

Measuring Accurately and Reproducibly

Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45 °C
Thorough mixing of samples in microplate wells by user-programmable Automix feature
Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader