PCR Polishing Kit, Agilent Technologies

Supplier: Agilent Technologies
200409 200409
CAST200409EA 421.2 CAD
ST200409 CAST200409
PCR Polishing Kit, Agilent Technologies
Nucleic Acid Reagents Nucleic Acid Modification and Labelling
PCR polishing kit increases blunt-end cloning efficiencies associated with PCR-generated fragments.

  • Generates blunt-ended DNA fragments in 30 minutes
  • More efficient than Klenow polishing
  • Optimizes blunt-end cloning efficiencies

PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.

Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.
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