pESC Yeast Epitope Tagging Vectors, Agilent Technologies

Supplier: Agilent Technologies
217451 217454 217452 217451 217455 217453 217452 217453 217455 217454
CA95040-810EA 994.16 CAD
CA95040-810 95040-810 99900-528 CA95040-812 99900-516 CA99900-530 95040-812 99900-530 CA99900-528 CA99900-516
pESC Yeast Epitope Tagging Vectors, Agilent Technologies
Vectors, Plasmids, Libraries and Inserts
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae and each vector contains GAL1 and GAL10 yeast promoters in opposing orientations.

  • Fast and easy immunoprecipitations

These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters. Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.

It contains DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies.

A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter.

These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR