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Specifications
- Description:Legacy ECM 2001 Electroporation System. Includes: Legacy ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm Package of 30 (10 each) and Cuvette Rack 660
- Power:100/115 V, Single Phase
- Fuse:7 A Slow Blow
- Dimensions:43W×27.9H×39.7D cm (17×11×151/2")
- Weight:21.3 kg (47 lbs.)
- Cat. no.:58018-010
Specifications
About this item
Multipurpose systems with AC wave for cell alignment and square DC wave for cell fusion and mammalian transfection.
- Wide range of voltages from 5 to 3000 V
- Pulse durations from 10 µsec to 10 sec
- Arc quenching
- Digital display of actual voltage and pulse length delivered
The Legacy ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation, and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, specialty electrodes, and other available accessories.
For cell fusion applications, the ECM 2001 is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Electrofusion is carried out by applying both the AC and DC pulse waveforms. Microsecond switch-over time from AC to DC allows for highly efficient fusion. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process, resulting in a higher number of hybrids.
The 1 MHz AC pulse aligns cells together in real time, saving time (seconds vs. hours) in comparison to PEG based cell fusion methods. For use in hybridoma production applications, the ECM 2001 generator, 3.2 mm gap or 10 mm gap microslides, and connection cables are recommended. This system does not require proprietary fusion medium, but can be used with Cytofusion medium C for convenience. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.
Utilizing the ECM 2001 for standard mammalian cell transfections is simple, the AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab.
Ideal for animal embryo cloning; hybridoma and quadroma production, plant protoplast fusion for transgenic plant generations, and electroporation of plant cells, mammalian cells, bacteria, yeast, and fungi.