About this item
Use these kits to maintain DNA that is otherwise unclonable
Kits are based on the linear cloning plasmids pJAZZ®, which are not subject to supercoiling in the cell. The ends of the pJAZZ® vectors can rotate freely as the molecule is replicated, as it is not placed under the torsional stress typically associated with supercoiling plasmid DNA. The result is the ability to clone numerous classes of structure-rich, highly stable sequences. Each BigEasy® kit is convenient to use, containing pre-cut, dephosphorylated pJAZZ® cloning vector with chloramphenicol or kanamycin resistance, DNATerminator® end repair enzymes and buffer, ligase and ligation buffer containing ATP, sequencing primers, competent cells, and DNA controls. Kits are available with 5, 10, or 20 reactions.
The kits are ideal for constructing shotgun libraries with large inserts up to 30kb or for cloning smaller products, particularly in instances where the target DNA is difficult to clone in conventional vectors.
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- • State issued document with your organization's Federal Tax ID Number
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- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.