Immunoprecipitation Starter Pack, Cytiva
Catalog #: CA95017-186L
Supplier: Avantor
Discontinued
Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
Specifications
- pH range:3 - 9
- Description:Immunoprecipitation Starter Pack
- Kit contents:Protein A Sepharose 4 Fast Flow (1 x 2 mL), Protein G Sepharose 4 Fast Flow (1 x 2 mL) and Instructions
- Matrix:Highly cross-linked agarose, 4%
- Ligand:Native Protein A (nProtein A), Recombinant Protein G Lacking Albumin-binding Region (Protein G)
- Binding capacity:>35 mg Human IgG/mL medium for nProtein A, >24 mg Human IgG/mL medium for Protein G
- Ligand density:~6 mg nProtein A/mL, ~2 mg Protein G/mL
- Ligand coupling method:Cyanogen Bromide Activation
- Cat. no.:CA95017-186L
Specifications
About this item
Immunoprecipitation is a highly specific and effective technique for analytical separations of target antigens from crude cell lysates.
- High binding capacity with minimal nonspecific adsorption
- Low leakage
The proteins are immobilized on Sepharose 4 Fast Flow by the cyanogen bromide method. Protein A and protein G have different binding selectivities depending on the origin of the IgG.
In combination with other techniques such as SDS-PAGE and immunoblotting immunoprecipitation can detect and quantify antigens, determine relative molecular weights, monitor protein turnover and posttranslational modifications, and check for enzyme activity.
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