About this item
- Eliminates ethidium bromide hazards
- Time savings
- Streamlines PCR sample handling and processing
Ready PCR Mix, 2X, offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. All components for assembly and performance of PCR reactions as well as loading and visualization of PCR products on agarose gels are included. The user supplies primer and template DNA.
Ready PCR Mix is supplied as a 2X mixture of reaction buffer, VWR® Extender™ DNA polymerase blend, dNTPs, electrophoresis tracking dye and a non-mutagenic EZ-Vision® visualization dye. Once amplification is complete, the PCR reaction can be directly loaded and separated on an agarose gel using the magenta-colored tracking dye (migrates at approximately 10bp on a 1% gel) to monitor the migration. After electrophoresis the PCR products are immediately visualized with standard UV illumination without additional post-run staining and destaining steps. VWR® Extender™ PCR-to-Gel Master Mix, 2X contains the same formulation as Ready PCR Mix, 2X without the fluorescent DNA Dye.
Ready PCR Mix includes all reagents except template and primer DNA.
Extender Master Mix includes all reagents except fluorescent DNA Dye, DNA template and primer DNA.
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- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.