Well-Coated™ Sulfhydryl Binding for Protein/Peptide Binding, G-BiosciencesSupplier: G-Biosciences
786-780 786-780 786-755 786-781 786-781 786-755
CA89168-034EA 637.29 CADCA89168-034 CA89167-982 89168-032 89167-982 89168-034 CA89168-032
Well-Coated™ Sulfhydryl Binding for Protein/Peptide Binding, G-Biosciences
Microplates HTS Microplates
G-Biosciences' Well-Coated™ Sulfhydryl Binding plates are designed to specifically bind free sulfhydryls of peptides, proteins and other molecules
G-Biosciences' Well-Coated™ Sulfhydryl Binding plates are maleimide activated plates that react with free sulfhydryls to form stable thioether bonds at pH 6.5-7.5. pH >7.5 significantly increases the reaction of amines with the maleimide groups.
The wells are coated to a 100µL depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. We recommend the use of Ellman’s Reagent to determine the amount of free sulfhydryls present on your peptdie, protein or ligand. For reducing agents to reduce disulfide bridges to generate free sulfhydryls click here. For peptides or proteins lacking sulfhydryls, SATA (N-Succinimidyl-S-acetylthioacetate) or Traut’s Reagent (2-Iminothiolane hydrochloride) can be used to add sulfhydryls via amine modification.
The Well-Coated™ Sulfhydryl Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for binding assays.
Ordering information: Accessories available.