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E.Z.N.A.® Blood DNA Extraction Kits

Supplier: OMEGA BIO-TEK CA
E.Z.N.A.®
D2492-00 M6221-00 M6211-00 M6221-01 M6211-01 D3494-04 D5032-00 D3494-00 D1192-00 D5032-03 D0714-50 D0714-640
Omega Bio-tek provides various methods for genomic DNA extraction from whole blood and body fluid, buffy coat, serum, plasma, saliva, cultured cells, buccal swabs and other body fluids

The Mag-Bind® Blood DNA systems are used for preparation of genomic DNA from buffy coat, serum, plasma, saliva, cultured cells, buccal swabs and other body fluids using paramagnetic beads. Magnetic beads can be used in 24, 96 or 384 well formats to fit your sample size to process manually or with automation. By eliminating retention volume commonly seen with filter based applications magnetic beads allow for higher recovery and optimal yield. Magnetic stands can be added to liquid handlers which utilize SBS format and can be programmed for your application allowing simultaneous processing of multiple plates. The technology allows for higher purity and flexible elution volumes. The Mag-Bind® Blood DNA Kits are compatible with open formatted instruments such as Hamilton Nimbus, Starlet, Star Plus, Tecan Freedom Evo®, Beckman Coulter Biomek 2000®, 3000®, NX®, FX®, and Thermo Kingfisher Flex®, Kingfisher® Duo.

These solution-based SQ Blood DNA systems allow versatility in amount of starting material and extraction of high-molecular weight DNA. The SQ Blood DNA Kit I lyses and removes red blood cells first and subsequently lyse white blood cells to release mitochondrial and genomic DNA. The SQ Blood DNA Kit II method first lyses the outer cell membrane followed by lysis of the nuclei to release genomic DNA. The SQ Blood DNA systems purify and concentrate released DNA via alcohol precipitation.

The E.Z.N.A.® Blood DNA systems use silica membrane technology accompanied by a specialized buffer system to purify DNA from 1 – 250 µL blood with the Blood DNA Kit, 250 μL - 2 mL blood with the Blood DNA Midi Kit, and 2 – 10 mL blood with the Blood DNA Maxi Kit. High-throughput DNA extraction from 1 – 250 µL can be performed using a 96-well, silica membrane method with the E-Z 96 Blood DNA Kit.




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Description Format Kit Size Sample Size Sample Type Supplier No. VWR Catalog Number Unit Price Quantity
Blood DNA Maxi Kit Maxi Spin Column 2 Preps 2 mL - 10 mL Blood D2492-00 CA101414-378 Each Retrieving Restricted
Blood DNA Midi Kit Midi spin columns 100 Preps 250 μL - 2 mL Blood D3494-04 CA101319-042 Each Retrieving Restricted
Blood DNA Midi Kit Midi spin columns 2 Preps 250 μL - 2 mL Blood D3494-00 CA101414-388 Each Retrieving Restricted
E-Z 96® Blood DNA Kit Silica Filter Plate 1 x 96 Preps 10 - 250 μL Blood D1192-00 CA101414-368 Each Retrieving Restricted
SQ Blood DNA Kit Solution Based 300 mL Scalable Blood D5032-03 CA101319-064 Each Retrieving Restricted
SQ Blood DNA Kit Solution Based 10 mL Scalable Blood D5032-00 CA101319-058 Each Retrieving Restricted
SQ Blood DNA Kit II Solution Based 50 mL Scalable Blood D0714-50 CA101414-338 Each Retrieving Restricted
restricted You must log in to order restricted items. We request that you provide the required business documentation to purchase this product for the first time.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • issued document with your organization's Federal Tax ID Number
  • Government issued document with your organization's Resale Tax ID Number
  • Any other Government ID that includes the business name and address


VWR will not lift restrictions for residential shipping addresses.

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SPECIFICATIONS

Temperature range –80 to 121°C
Processing range 25 µL to 1.5 mL

ACCESSORIES

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Accessories
Description Kit Size No. of Reactions Size Format Concentration Supplier No. VWR Catalog Number Unit Price Quantity
2 x PCR TaqMixtures - 400 5 x 1 mL - - TQ2200-01 CA101414-944 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

2X TaqMastermix 0.5 mL - - - - TQ2200-00 CA101414-942 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BL Buffer 100 mL - - - - PD062 CA101414-704 Each (100ml) Retrieving Restricted
dNTP Set, 100 mM (4 x 250 µL) - - - Set 100 mM TQAC135 CA101414-958 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

dNTP Set, 25 mM (1 mL) - - - Mix 25 mM TQAC136 CA101414-960 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Elution Buffer 100 mL - - - - PDR048 CA101414-778 Each (100ml) Retrieving Restricted
E-Z 96® Vacuum Manifold - - - - - VAC-03 CA101414-962 Each Retrieving Restricted
HiBind DNA columns 200 - - - - DNACOL-02 CA95043-212 Each Retrieving Restricted
HiBind DNA columns 50 - - - - DNACOL-01 CA95043-210 Each Retrieving Restricted
Magnetic Separation Device-B - - - - - MSD-02 CA101414-700 Each Retrieving Restricted
Proteinase K, >600 mAU/mL, Solution - - 10 mL - - AC116 CA101414-310 Each (10ml) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

RNase A (10 mg/mL) 5 mL - - - - PD090 CA101414-760 Each (5ml) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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